Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_K628001:Design

Designed by: Charles Thompson   Group: iGEM11_St_Andrews   (2011-09-19)


antimicrobial peptide kill switch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 10505
    Illegal suffix found in sequence at 5713
    Illegal XbaI site found at 387
    Illegal SpeI site found at 180
    Illegal PstI site found at 1152
    Illegal PstI site found at 2469
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 10505
    Illegal NheI site found at 125
    Illegal NheI site found at 1311
    Illegal SpeI site found at 180
    Illegal SpeI site found at 5714
    Illegal PstI site found at 1152
    Illegal PstI site found at 2469
    Illegal PstI site found at 5728
    Illegal NotI site found at 139
    Illegal NotI site found at 5721
    Illegal NotI site found at 5758
    Illegal NotI site found at 10511
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 10505
    Illegal BamHI site found at 65
    Illegal BamHI site found at 5740
    Illegal XhoI site found at 10472
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 10505
    Illegal suffix found in sequence at 5714
    Illegal XbaI site found at 387
    Illegal SpeI site found at 180
    Illegal PstI site found at 1152
    Illegal PstI site found at 2469
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 10505
    Illegal XbaI site found at 387
    Illegal XbaI site found at 10520
    Illegal SpeI site found at 180
    Illegal SpeI site found at 5714
    Illegal PstI site found at 1152
    Illegal PstI site found at 2469
    Illegal PstI site found at 5728
    Illegal NgoMIV site found at 4792
    Illegal NgoMIV site found at 5146
    Illegal NgoMIV site found at 5306
    Illegal NgoMIV site found at 5674
    Illegal NgoMIV site found at 6101
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1437
    Illegal BsaI site found at 1615
    Illegal BsaI site found at 2643
    Illegal BsaI site found at 9099
    Illegal BsaI.rc site found at 277
    Illegal BsaI.rc site found at 368
    Illegal BsaI.rc site found at 1077
    Illegal SapI.rc site found at 3724
    Illegal SapI.rc site found at 8077
    Illegal SapI.rc site found at 10181


Design Notes

TBA


Source

It comes from pigs. Again, I will update soon.

References